Fig. 3.

Downregulation of CNA-α and CNA-β subunits gene expression following endogenous transcription of small interfering RNA (siRNA) encoded by exogenous cDNA templates. cDNA templates were delivered efficiently by adenovirus vector to all cells in culture. In the figure, siCNA refers to silenced CN A subunit. Reduction of CNA β (A) and α (B) subunit transcript levels is produced by infection with adenovirus vectors carrying specific siRNA and not by control virus (U6). Luciferase expression (C) is reduced by either CNA α or β subunit but not by the control virus (U6). Data are means ± SD from n = 3 preparations. Statistical significance was determined by ANOVA. *Significance (P < 0.05) versus control.