Fig. 6.

Phospholamban (PLB) (A) and Na⁺/Ca²⁺ exchanger 1 (NCX1) (B) transcript levels in control myocytes or following exposure to INCA-6 or CsA and PLB Western blot (C). INCA-6 (5 μM) was added, with or without TG (10 nM), 1 day after initial plating, and the myocytes were harvested 3 days thereafter. PLB and NCX1 transcripts levels were measured by real-time PCR as described above. Data are means ± SD from n = 3 preparations. Statistical significance was determined by ANOVA. *Significance (P < 0.05) versus control; #significance (P < 0.05) versus TG. C: Western blots showing PLB protein levels and state of oligomerization in control myocytes or following 7 days exposure to 10 nM TG.