Fig. 5.

Combined effect of inhibition of all the MAPK pathway stability of IL-8 mRNA in CF cells. IB3-1 cells (A) or IB3-1-TTP cells (B) were treated with the indicated combination of the chemical inhibitor SB-203580 (10 μM), U0126 (10 μM), and SP-600125 (10 μM) for the MAPK signaling pathways. RNA was isolated from IB3-1 and IB3-1-TTP cells after actinomycin D treatment for the indicated time intervals, and the remaining mRNA was analyzed by quantitative real-time PCR. The data reflect averages of at least 3 independent experiments (*P < 0.05 and **P > 0.05).