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. 2010 Oct 15;300(1):L81–L87. doi: 10.1152/ajplung.00051.2010

Fig. 6.

Fig. 6.

Proteasome function coordinates with MAPK pathways to modulate the stability of IL-8 mRNA in CF cells. IB3-1 cells (A) or CFBE (B) or IB3-1-TTP cells (C) were treated for 2 h with MG-132 (10 μM) followed by incubation with the indicated combination of the chemical inhibitor SB-203580 (10 μM), U0126 (10 μM), and SP-600125 (10 μM) for the MAPK signaling pathways. RNA was subsequently isolated from IB3-1 and IB3-1-TTP cells after actinomycin D treatment for the indicated time intervals, and the remaining mRNA was analyzed by quantitative real-time PCR. The data reflects averages of at least 3 independent experiments (*P < 0.05 and **P > 0.05).