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. Author manuscript; available in PMC: 2011 Dec 21.
Published in final edited form as: Curr Biol. 2010 Dec 2;20(24):2159–2168. doi: 10.1016/j.cub.2010.11.015

Figure 1. Novel miRNAs identified in aged C. elegans.

Figure 1

(A) Secondary structures of putative precursor hairpins corresponding to miRNAs identified in a pool of RNA enriched for aged C. elegans. The predicted mature sequence is highlighted in red.

(B) Conservation of novel miRNAs miR-A, miR-B, miR-C and miR-I to known miRNAs. Identical nucleotides are highlighted in black.

C) Validation of expression of candidate novel miRNAs. The expression of novel miRNAs mir-G, mir-H, mir-I and mir-K was confirmed by TaqMan qRT-PCR in wild-type (N2) C. elegans. Consistent with their classification as miRNAs, their expression was significantly reduced in alg-1(gk214) mutant animals. The expression of the miRNA let-7 is shown as a positive control. The expression of a non-miRNA small RNA species (snoRNA sn2429) does not change in alg-1(gk214) background (DNS). *, p < 0.05, alg-1(gk215) compared to wild-type (N2) (Student's t-test).