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. 2010 Nov 3;105(1):279–292. doi: 10.1152/jn.00747.2010

Fig. 4.

Fig. 4.

Examples of Class 2 and Class 1 responses from cells in layers 2/3 and layer 4 of A1. A: Class 2 response in layers 2/3. Ai: the cell responded with paired-pulse facilitation to MGBv stimulation at 10 Hz. Increasing stimulation intensities produced increases in EPSP amplitudes. Aii: stimulation at 10 Hz (250 μA), in the presence of ionotropic glutamate receptor antagonists (DNQX and AP5), failed to produce any EPSPs. Aiii: HFS (125 Hz, 200 μA) in the presence of DNQX and AP5 produced a slow and prolonged membrane depolarization (blue trace) that could be blocked with a cocktail of type 1 (LY367385) and type 5 (MPEP) metabotropic glutamate receptor antagonists (black trace). B: Class 1 response in layers 2/3. Bi: this cell responded with paired-pulse depression to MGBv stimulation at 10 Hz. EPSP amplitude was unaffected by increases in stimulation intensities. Bii: stimulation at 10 Hz (250 μA), in the presence of DNQX and AP5, failed to produce any EPSPs. Biii: HFS (125 Hz, 250 μA) in the presence of DNQX and AP5 did not produce any membrane potential changes. C: Class 1 response in layer 4. Ci: this cell responded with paired-pulse depression to MGBv stimulation at 10 Hz. EPSP amplitude was unaffected by increases in stimulation intensities after a threshold was reached. Cii: stimulation at 10 Hz (250 μA), in the presence of DNQX and AP5, failed to produce any EPSPs. Ciii: HFS (125 Hz, 200 μA) in the presence of DNQX and AP5 did not produce any membrane potential changes. Arrows represent timing of stimulation for all 10 Hz trials, and black bars represent the duration of stimulation in HFS trials. Excluding HFS trials, all traces represent the average of 10 sweeps. Scale bars in Ai, Bi, and Ci apply to Aii, Bii, and Cii, respectively.