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. 2010 Nov 17;105(1):321–335. doi: 10.1152/jn.00332.2010

Fig. 4.

Fig. 4.

TMEM16A and SV2 labeling colocalize at photoreceptor terminals. A: immunolabeling of TMEM16A (green) in a vertical retinal section. B: immunolabeling of the same retinal section with a synaptic vesicle marker, SV2 (red). Autofluorescence in the outer segments was observed without secondary antibodies. C: merged fluorescent and DIC image of TMEM16A and SV2. D: brightfield DIC image of a rod photoreceptor lacking its outer segment immunolabeled with TMEM16 (green). E: brightfield DIC image of the same rod immunolabeled with SV2 (red). F: merged brightfield DIC image with both TMEM16A and SV2. G–I: magnified images of the same rod terminal showing that TMEM16A overlaps completely with SV2. J–L: brightfield DIC images of a small single cone photoreceptor immunolabeled with TMEM16A (green, J), SV2 (red, K), or both (L). M–O: magnified images of the same cone terminal showing that TMEM16A overlaps completely with SV2. OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Synaptic terminal regions are indicated by the arrows in D and J. Scale bar is 10 μm.