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. 2011 Feb;25(2):590–599. doi: 10.1096/fj.10-167304

Figure 4.

Figure 4.

Stratification of colorectal tumors by abundance of GIV-fl expression as determined by IHC. A) Intensity of GIV-fl staining varied among tumors. Paraffin-embedded human colon cancer samples of Duke's clinical stage B2 were analyzed for GIV-fl as in Fig. 3A. Staining of the tumor epithelium was scored as 0 = absent (a), 1+ = moderately positive (b), 2+ = strongly positive (c) by 3 independent observers masked to patient outcome and stage. Images are representative of tumors in each scoring category. B) Kaplan-Meier survival curves for patients with colorectal cancer stratified according to GIV-fl expression status. GIV-fl expression was determined in 56 patients with Duke's B2 colon cancers as in Fig. 3A. Staining was scored as negative or positive by 3 independent observers masked to patient outcome and stage with >95% congruency. Log-rank analysis of the survival curve shows that survival of patients with colorectal cancer where tumor epithelium stained negative for GIV-fl protein (GIV-fl neg) was significantly better than that of patients with cancers where tumor epithelia stained positive GIV-fl (GIV-fl pos) by IHC using GIV-CTAb (P<0.001). C) Kaplan-Meier survival curves for patients with colorectal cancer, stratified according to intensity of GIV-fl expression. GIV-fl expression was determined in 56 patients with Duke's B2 colon cancers and scored as in A. Log-rank analysis of the survival curve shows a trend that among GIV-fl-positive tumors, higher GIV-fl staining (2+ vs. 1+) correlated with worse prognosis, but it did not reach significance (P=0.69).