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. 2011 Feb;25(2):600–612. doi: 10.1096/fj.10-167502

Table 2.

Electron transport chain subunit enzyme activities

Genotype CS (mU · mg−1) NCR (mU · mg−1) Complex III (mU · mg−1) Complex IV (s−1 · mg−1)
Wild type 2052 ± 178 2169 ± 98 5222 ± 264 576 ± 80
Sphk2−/− 1836 ± 134 2212 ± 295 5667 ± 512 669 ± 33

Citrate synthase (CS), NCR (complexes I and III), ubiquinol:ferricytochrome-c oxido-reductase (complex III), and COX (complex IV) activities were measured in frozen-thawed, cholate-solubilized heart mitochondria at 37°C. Values are expressed as means ± se; n = 3/group.