Figure 1.

Inhibition of JNK promotes translocation of E-cadherin and β-catenin to the cell-cell adhesion sites and formation of actin bundles. Human primary keratinocytes were treated with 10 μM SP600125 for 30 min or transduced with recombinant lentivirus encoding for flag-JNK1DN. A) Cell lysates were subjected to Western blotting for E-cadherin, β-catenin, Flag, and β-actin. B–E) Confocal (B, D) and lateral images (C, E) of cells stained for β-catenin (red) and E-cadherin (green; B, C) or phalloidin (green; D, E). Cell nuclei were stained with Hoechst (blue). Lateral images were formed by maximum intensity projection from confocal z-stack images. View: ×63. Scale bars = 20 μm.