Figure 2.

α-Catenin translocates to cell-cell contact sites on inhibition of JNK. Human primary keratinocytes remained untreated (ctrl), they were treated with SP600125 (SP600125), or they were genetically modified to express dominant-negative JNK1 (JNK1DN). A) Confocal images of the cells stained with α-catenin (red) and actin phalloidin (green). Cell nuclei were stained with Hoechst (blue). Scale bars = 20 μm. B) Lateral images were formed by maximum intensity projection from confocal z-stack images. C) Human primary keratinocytes were transduced with recombinant lentivirus encoding for α-catenin-DsRed. On SP600125 (20 μM) treatment, α-catenin-DsRed-expressing cells were followed using time-lapse microscopy. View: ×63.