Figure 5.

α-Catenin is necessary for JNK-mediated regulation of AJs. A, B) Human primary keratinocytes were transduced with recombinant lentivirus encoding for shRNA targeting the α-catenin mRNA (si-α-cat; A); ME180 cells lacking α-catenin were transduced with full-length a-catenin (α-cat-M180; B). Amounts of E-cadherin, β-catenin, and α-catenin were detected using Western blotting. C–F) Confocal (C, E) and lateral images (D, F) of si-α-cat cells (C, D) or ME180 and α-cat-M180 cells (E, F) treated with either vehicle (ctrl) or SP600125 (10 μM) for 30 min, then stained with β-catenin (red) and actin phalloidin (green); nuclei were stained with Hoechst (blue). Lateral images were formed by maximum-intensity projection from confocal z-stack images. View: ×63. Scale bars = 20 μm.