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Binding of α-catenin to β-catenin and actin is required for JNK-mediated AJ formation. A) ME180 cells expressing different domains of α-catenin, as indicated, were treated with either vehicle (ctrl) or SP600125 (10 μM) for 30 min. Confocal images of cells stained with β-catenin (red) and actin phalloidin (green); nuclei were stained with Hoechst (blue). View: ×63. Scale bars = 20 μm. B) Lateral images were formed by maximum intensity projection from confocal z-stack images.
