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. 2011 Feb;25(2):727–736. doi: 10.1096/fj.10-173682

Figure 2.

Figure 2.

PC Kcne3 is selectively up-regulated in remodeling arising from targeted deletion of Kcne2. A) qRT-PCR analysis of remodeling of the fundic Kcne expression profile by targeted deletion of Kcne2; mRNA expression level expressed as a ratio to that of reference gene GAPDH; n = 10 mice/genotype/gene. B) Representative agarose gel of cDNA fragments for Kcne3 and the reference gene HPRT after semiquantitative RT-PCR from gastric mucosal lysates from Kcne2+/+ and Kcne2−/− mice as indicated. C) Mean band optical densities from samples as in panel B; n = 4 independent preps (and mice)/genotype. D) Representative Western blot of Kcne3 protein in membrane fractions from Kcne2+/+ and Kcne2−/− mouse fundus preparations as indicated, using anti-Kcne3 antibody. Migration distance of molecular mass markers is indicated at left. Arrows indicate expected sizes of nonglycosylated and fully glycosylated (mature) Kcne3. E) Band optical density from Kcne3 (mature form) Western blots of Kcne2+/+ and Kcne2−/− gastric mucosal membrane fractions, as in panel D; n = 7 independent preparations from 3–5 stomachs each; total of 21–35 stomachs/genotype. Error bars = sem.