Figure 2.

Confocal microscopy detection of adenosine-induced O₂⁻ and H₂O₂ generation. (A) Application of exogenous adenosine induced concentration-dependent increase in the generation of O₂⁻ as determined by the change in average intensity of the hydroethidine (HE) fluorescence signal under equal time interval in cerebral arterial segments that started at 10 nmol/L and significantly increased by subsequent graded increase in adenosine concentration and reached maximum at 1 μmol/L. (B) Confocal microscopy images of cerebral arterial segment showing adenosine (1 μmol/L) induced O₂⁻ generation and gradual formation of H₂O₂ as determined by the combined use of the O₂⁻ detecting probe HE, and the H₂O₂ detecting probe dichlorofluorescien-diacetate (DCF-DA) (n=8, ^*P<0.05).