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. 2010 Mar 10;30(10):1742–1755. doi: 10.1038/jcbfm.2010.36

Figure 2.

Figure 2

The BCRP-mediated BODIPY FL prazosin transport in isolated brain capillaries. (A) Representative image of an isolated rat brain capillary at steady state after 1 h exposure to 2 μmol/L BODIPY FL prazosin. The image analysis of the cross-section (gray) is shown below the image. Note that BODIPY FL prazosin (BP) transport is concentrative from bath (no visible fluorescence) to endothelium (low fluorescence) to capillary lumen (high fluorescence). (B) The BCRP inhibitor, FTC, blocks concentrative BODIPY FL prazosin transport. Analysis of the cross-section (gray) shows fluorescence in the endothelial cells but no accumulation of BODIPY FL prazosin in the capillary lumen. (C) Time course of BODIPY FL prazosin accumulation in capillary lumens. Each data point represents the mean±s.e.m. for 10 to 15 capillaries from a single preparation (pooled tissue from 10 rats). Units are arbitrary fluorescence units (scale 0 to 255). BCRP, breast cancer resistance protein; FTC, fumitremorgin C.