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. 2010 Mar 10;30(10):1742–1755. doi: 10.1038/jcbfm.2010.36

Figure 4.

Figure 4

The BCRP expression and function in isolated rat brain capillaries is gender independent. (A) In female rats, BCRP monomer and dimer expression in kidney and BCRP dimer expression in liver are reduced compared with male rats. (B) BCRP and P-glycoprotein expression in brain capillaries from male and female rats is gender independent. For all western blots, β-actin was used as protein loading control. (C) BCRP-mediated transport of BODIPY FL prazosin (BP) into the lumens of capillaries from male and female rats is gender independent in untreated control capillaries and FTC-treated capillaries. For luminal BODIPY FL prazosin fluorescence, each data point represents the mean±s.e.m. for 10 to 15 capillaries from a single preparation (pooled tissue from 10 rats). Units are arbitrary fluorescence units (scale 0 to 255). Statistical comparison: ***significantly lower than controls, P<0.001. BCRP, breast cancer resistance protein; FTC, fumitremorgin C.