FIGURE 5.

BIC/miR-155 is up-regulated and CD10 is down-regulated upon treatment of BJAB cells with LPS. BJAB cells were treated with 5 μg/ml of LPS for the indicated amounts of time (h). A, RT-PCR was performed for BIC, REL, and GAPDH transcripts on RNA samples obtained after treatment of BJAB cells with LPS. For each time point, RT-PCR from three independent samples are shown. B, micro-RNA-specific real time PCR was performed to assess the levels of miR-155 in BJAB cells. Values were normalized to the 5S rRNA levels and represent the mean ± S.E. of three experiments performed with three samples. C, RT-PCR was performed using primers for CD10 or GAPDH. For each time point, RT-PCR from three independent samples are shown. D, Western blotting was performed on whole cell extracts using antibodies against CD10 and β-tubulin. E, BJAB cells were treated with LPS for 72 h and nuclear extracts were analyzed by EMSA using the PU-1 site from the CD10 promoter (see Fig. 2B).