FIGURE 2.

Lateral organization of the MAYV envelope and the effect of cholesterol depletion on this parameter. HCCPs (A) and LCCPs (B) were treated with 0, 10 or 25 mm MβCD and, after removal of the drug, incubated with Laurdan. Samples were then analyzed on a spectrofluorimeter at 37 °C using an excitation wavelength of 360 nm and scanning the fluorescence emission from 390 to 550 nm. Generalized polarization (GP) values from these samples were calculated as a function of the fluorescence emission intensities at 440 and 490 nm, as described under “Experimental Procedures” (C).