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KRIBB11 binds to HSF1. A, structure of biotinyl-KRIBB11. B, 300 μg of total protein was incubated with 10 μm biotinyl-KRIBB11 and competed with KRIBB11 at an indicated concentration. Proteins were captured with 10 μl of Dynabeads M-280 streptavidin and eluted by boiling in SDS-PAGE sample buffer. The eluted proteins were resolved by 10% SDS-PAGE and blotted with an anti-HSF1-specific antibody.
