FIGURE 4.

FTY720-P is released from hSPNS2-expressing cells. CHO/SPHK1 or CHO/SPHK2 cells were transfected with pEGFP (mock) or pEGFP/hSPNS2 (SPNS2). 24 h after transfection, the cells were incubated with releasing medium containing 5 μm FTY720 (A) or Sph (B) for 2 h. The releasing medium was collected, and the amounts of released FTY720-P (A) and S1P (B) were measured by HPLC as described under “Experimental Procedures.” Experiments were performed more than three times, and error bars indicate the S.D. N.D., not detected.