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. 2010 Nov 8;286(3):1884–1894. doi: 10.1074/jbc.M110.183053

TABLE 1.

Thermodynamic parameters obtained by fitting experimental data to AB or ABB hetero-association models (SEDPHAT)

Experiments 19–21 were analyzed using a two-site model (Microcal Origin) (N1 = 1, N2 = 4). The parameters shown for these experiments are apparent values.

No. SfbI construct Binding sitea FN fragment Modeb Kd ΔH −TΔS ΔG
nm kcal mol−1 kcal mol−1 kcal mol−1
1 SfbI-4 FN30kDa 166.09 −30.98 21.36 −9.62
2 SfbI-5 FN30kDa 1.42 −45.30 32.74 −12.56
3 SfbI-UR1 FN70kDa 3.30 −70.02 57.98 −12.04
4 SfbI-4 FN70kDa 37.44 −32.41 21.87 −10.54
5 SfbI-5 FN70kDa 2.53 −40.33 28.13 −12.20
6 SfbI-UR1 FN100kDa 1.85 −66.73 54.34 −12.39
7 SfbI-4 FN100kDa 32.00 −36.79 26.15 −10.64
8 SfbI-5 FN100kDa 1.77 −42.69 30.26 −12.42
9 SfbI-4 2–5FNI 304.69 −27.51 18.26 −9.25
10 SfbI-5 2–5FNI 12.96 −29.91 18.72 −11.19
11 SfbI-UR12 SfbI-UR1 FN70kDa S 0.07 −78.39 64.01 −14.38
11 SfbI-2 FN70kDa S 3.82 −38.90 26.95 −11.95
12 SfbI-UR1 FN70kDa C 0.09 −80.36 66.13 −14.23
12 SfbI-2 FN70kDa C 3.41 −32.62 20.60 −12.02
13 SfbI-UR1 FN100kDa S 0.06 −63.97 49.51 −14.46
13 SfbI-2 FN100kDa S 3.24 −27.55 15.50 −12.05
14 SfbI-UR1 FN100kDa C 0.36 −67.83 54.43 −13.40
14 SfbI-2 FN100kDa C 8.57 −25.32 13.87 −11.45
15 SfbI-45 SfbI-4 FN30kDa S 4.48 −32.41 20.56 −11.85
15 SfbI-5 FN30kDa S 0.32 −50.49 37.02 −13.47
16 SfbI-4 FN30kDa C 12.25 −37.42 26.19 −11.23
16 SfbI-5 FN30kDa C 0.05 −45.40 30.81 −14.58
17 SfbI-4/5 FN70kDa C 2.66 −41.51 29.34 −12.17
18 SfbI-4/5 FN100kDa C 1.55 −34.98 22.48 −12.50
19 SfbI-UR12345 SfbI-4 FN30kDa S (4) (−33.04) (21.12) (−11.92)
19 SfbI-5 FN30kDa S (0.05) (−47.25) (32.56) (−14.69)
20 SfbI-4/5 2–5FNI S (188) (−33.56) (24.01) (−9.55)
21 SfbI-UR1 FN100kDa S (0.16) (−105) (92) (−13)
21 SfbI-2 FN100kDa S (3.7) (−30.5) (18.5) (−12)

a Binding sites within multiple site constructs are assigned according to the expected order of binding based on single site experiments except for experiment 21 where it is based on Kd values obtained in experiments 13 and 18.

b C indicates ligand in cell; S indicates ligand in syringe.