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. 2010 Nov 8;286(3):1895–1902. doi: 10.1074/jbc.M110.164962

FIGURE 1.

FIGURE 1.

SsrBC binds upstream of the sifA, sifB, and sseJ promoters. A, promoters were mapped by primer extension on RNA from an hns null strain (+) and its ssrB null isogenic derivative (−) (14, 23). Gene-specific primers are indicated below each gel, and from left to right each panel contains C, T, A, and G sequencing ladders. The sequence to the right of each panel represents DNA flanking the start sites, which are indicated by boxed letters. The asterisk indicates a nonspecific primer extension product not observed with an alternate sifB primer. The sifB +1 coordinate was defined as the G position. B–D, DNase I footprinting (B) sifA, (C) sifB, and (D) sseJ. The flanking lanes of each panel contain DNase I-only ladders. Lanes containing SsrBC (indicated by the triangle below each panel) contain, from left to right, 25, 100, 200, 400, and 800 nm protein. The black bars indicate regions of protection, and predominant hypersensitive sites are represented by arrows. Binding coordinates are relative to the transcription start sites identified in A and were determined using DNA sequencing ladders as described previously (23).