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Dimerization of LIS1 is necessary for its function. A, schematic diagram of GST-LIS1, GST-ΔN-LIS1, and ΔN-LIS1, and SDS-PAGE gel image. B, MT gliding velocities of dynein in the presence of 1 mm ATP. The error bars indicate standard deviations. The numbers of MTs measured were 55 (Dynein), 68 (Dynein + ΔN-LIS1), 66 (Dynein + GST-ΔN-LIS1), and 61 (Dynein + GST-ΔN-LIS1 + NN). The p value was calculated using Student's t test (*, p < 0.001).
