Table 1.

Permeabilities of reconstituted proteoliposomes

Permeability	Conditions	Liposomes	GlpF	AqpZ	GlpF + AqpZ
Glycerol
kgly, s−1	Control at 5°C	0.019  ± 0.006	7.8  ± 0.7	0.011  ± 0.005	38  ± 1
	Control at 20°C	0.17  ± 0.02	18  ± 1	0.14  ± 0.02	54  ± 7
	HgCl2 at 20°C*	0.11  ± 0.02	0.28  ± 0.01	0.14  ± 0.02	16  ± 1†
Pgly, cm/s at 5°C		6.2  × 10−8	2.5  × 10−5	3.6  × 10−8	1.2  × 10−4
Ea, kcal/mol		27  ± 2	9.6  ± 1.5	25  ± 2	4.9  ± 1.5
Water
kw, s−1	Control at 5°C	2.4  ± 0.2	23  ± 3	154  ± 30
	Control at 20°C	13.5  ± 0.2	41  ± 1	158  ± 18
	HgCl2 at 20°C*	2.5  ± 0.2	4.8  ± 0.1†	98  ± 6
	HgCl2 + DTT at 20°C*	ND	32  ± 5†	ND
Pf, cm/s at 5°C		5.1  × 10−4	4.9  × 10−3	3.3  × 10−2
Ea, kcal/mol		16	7	3

^*Proteoliposomes were preincubated with 100 μM HgCl₂ for 30 min at 37°C. Reversal of HgCl₂ inhibition was achieved by incubation with 1 mM DTT at 37°C for 30 min. ND, not determined. 

^† This value corresponds to 30 μM HgCl₂.