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Kinetic constants of nucleotide incorporation opposite template CMP
| NTP | kpol | Kd | kpol/Kd | Discriminationa |
|---|---|---|---|---|
| s−1 | μm | μm−1s−1 | ||
| GTP | 18 ± 1 | 275 ± 52 | 0.065 | 1 |
| UTP | 0.0030 ± 0.0006 | 1570 ± 820 | 0.0000019 | 34,000 |
| ATP | 0.0013 ± 0.0004 | 1180 ± 1000 | 0.0000011 | 59,000 |
| CTP | 0.00053 ± 0.00011 | 1100 ± 600 | 0.00000048 | 135,000 |
a Discrimination is the ratio of the specificity for a correct nucleotide (GTP in this case) to that of an incorrect nucleotide.
