FIGURE 2.

a, SDS-PAGE of the supernatant and protein pellet obtained after centrifugation of the fibrillogenesis mixtures containing 100 μm β2-m and 300 μm of each tetracycline congener. Samples were centrifuged after 48 h of incubation. Alongside the molecular mass standards, supernatant (s) and pellet (p) are shown for 4-epi-oxytetracycline (lane 1), anhydrochlortetracycline (lane 2), minocycline (lane 3), demeclocycline (lane 4), doxycycline (lane 5), and 4-epianhydrotetracycline (lane 6), respectively. Treated and non-treated β2-m are shown as controls. b, the percentage of protein in both supernatant and pellet of samples with and without tetracyclines. Proteins within each lane were quantified by densitometric analysis, and the percentage was determined as compared with control β2-m samples.