FIGURE 7.

a and b, ¹H-¹⁵N HSQC spectra of β2-m 0.65 mm in TFE 18% (phosphate 70 mm, NaCl 100 mm) at 37 °C recorded without (a) or with (b) doxycycline (1.96 mm). The spectra were obtained using the same acquisition conditions from two samples prepared using the same β2-m mother solution, with a 10.0-μl addition of 0.100 m doxycycline for b. The experimental data were treated with the same processing parameters and at the same absolute scaling (TOPSPIN, Bruker). The reported maps were drawn starting at the same lowest contour level and with the same level numbers and spacing.