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. 2010 Oct 11;286(3):2132–2142. doi: 10.1074/jbc.M110.148395

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 3. — DACH1 reduces the proportion of CD24^low/CD44^high cells. (A) Mammosphere assays of Met-1 cells transduced with vector control or DACH1. (B) FACS based CD24/CD44 double staining of Met-1 cells in vitro expressing DACH1 or control vector. (C) The cellular potential of CD24^low/CD44^high versus CD24^high/CD44^high cells was determined. Schematic representation of methods for analysis of Met-1 population. (D) FACS analysis. (E) Mammosphere formation and (F) tumor growth in nude mice studies comparing CD24^low/CD44^high and CD24^high/CD44^high cells.