FIGURE 4.

Endogenous DACH1 inhibits the stem cell phenotype. A, knocking down endogenous Dach1 expression by lentivirus shRNA vector to Dach1. B, FACS-based CD24/CD44 double staining and mammosphere assay of Met-1 cells expressing shCTL or shDACH1. C, mammosphere number and size of Met-1 cells expressing shDACH1. Data are mean ± S.E. of >5 spearate experiments. D, FACS display of CD24/CD44 double staining of MCF10A and Myc transformed MCF10A with vector control or DACH1. Western blot of MCF10A-transduced cells. Each cell type also expresses GFP from the vector as a marker.