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A, N41 cells transfected with the Lepr-b::eGFP overexpression vector and treated with leptin. B, immunohistochemistry showing the arcuate hypothalamic region adjacent to the third ventricle from mice administered leptin peripherally. C, fasted mice; D, mice administered leptin and cathepsin L inhibitor I peripherally. Cilia are stained with an Adenylyl cyclase III (AcIII)-specific antibody.
