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. 2010 Nov 16;286(3):2224–2235. doi: 10.1074/jbc.M110.149880

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© 2011 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 2. — Effect of THGP on ROMK currents. A, representative whole cell currents from Xenopus oocytes injected with ROMK2 (left) or THGP (middle) or both (right) are shown. B, macroscopic current-voltage relationships and C, currents activated at −100mV were plotted. D, Xenopus oocytes injected with HA-tagged ROMK2 alone or together with THGP were assayed for surface expression, as determined by a cell surface luminescence assay. E, corresponding Western blots of HA-tagged protein in total oocyte lysates. Water-injected oocytes were taken as control to determine antibody specificity (n = 15, ***, p < 0.0001 versus ROMK2).