FIGURE 1.

Toca-1 co-localizes with Arp2/3 complex within lamellipodia. A, A431 cells plated on gelatin-coated coverslips were serum-starved overnight and treated with EGF (100 ng/ml) for 5 min prior to fixation and IF staining with mouse anti-Toca-1 (green) and F-actin (red). Representative confocal micrographs are shown for green and red and a merge of both channels. The arrows indicate membrane ruffles, and boxed areas in a–c are shown below at higher magnification (d–f). Scale bar, 30 μm. B, representative confocal microscopy images are shown for IF staining of GFP-Toca-1 (green; a) in transfected A431 cells forming lamellipodia, together with staining for F-actin (red; b) and endogenous p34-Arc (magenta; c). The merged image indicates overlay of green, magenta, and red channels, with co-localization indicated by white color. Higher magnification views (e–h) of the boxed areas of the images are shown below. Scale bar, 30 μm.