FIGURE 2.

EGF induces Toca-1 phosphorylation and binding to N-WASP and Abi1. A, A431 cells were serum-starved overnight and treated with or without EGF (100 ng/ml) for the times indicated in minutes. SCL were subjected to IP with mouse anti-Toca-1 or a control mouse IgG (Ctl IP) prior to IB with antiserum specific for phosphotyrosine (pY), Toca-1, and N-WASP. Positions of molecular mass markers are shown on the left. B, A431 cells were serum-starved and treated with or without EGF (100 ng/ml) for 5 min. SCL were subjected to IP with rabbit anti-Abi1 or a control rabbit IgG and IB with mouse anti-Toca-1 and mouse and Abi1. Positions of molecular mass markers are shown on the left.