Deviation of NLC from the simple two-state Boltzmann model. NLC was measured in the presence of 0.5 mm salicylate. The NLC data were analyzed by the simple two-state Boltzmann model (Equation 3, A), the three-state model (Equation 4, B), and the two-state-Csa model (Equation 5, C). Solid lines show the fitting curves. Improvements of the fits are visually obvious in the residual plots by the three-state fitting (B) and the two-state-Csa fitting (C) compared with that by the simple two-state fitting (A). Indeed, >1013 times higher Akaike's weights were obtained for the three-state and the two-state-Csa fittings over the simple two-state fitting. Akaike's weights for the three-state and two-state-Csa were 0.19 ± 0.08 and 0.81 ± 0.08% (n = 8), respectively.