FIGURE 2.

Insulin-stimulated MARCKS phosphorylation in endothelial cells. Shown here are the results of dose response (A–C) and time course (D–F) experiments exploring insulin-mediated MARCKS phosphorylation responses in endothelial cells. Lysates prepared from insulin-treated endothelial cells were resolved by SDS-PAGE and analyzed in immunoblots probed with antibodies as shown against total or phosphorylated MARCKS (pMARCKS), or antibodies directed against total or phosphorylated Akt (pAkt). In the time course experiments, cells were treated with 100 nm insulin; in the dose response experiments, cells were analyzed 15 min after addition of insulin. A and D, representative immunoblots; B and C, and E and F present quantitative plots derived from pooled data; each point in the graphs represents the mean ± S.E. of four independent experiments that yielded similar results.