FIGURE 7.

Bz-IAA and Bz-NAA did not affect subcellular trafficking of PIN proteins. A, Arabidopsis root expressing PIN2-GFP fusion protein was incubated in medium containing 10 μm alkoxy-auxin or 25 μm TIBA for 30 min and then 50 μm BFA was added to the same medium to relocate a PIN2-GFP protein into the BFA bodies. B, the Arabidopsis PIN2-GFP line was incubated for 2 h in medium containing 50 μm BFA to induce BFA bodies, and the root was then treated with 10 μm alkoxy-auxin or 25 μm TIBA for 2 h in the medium. GFP fluorescent was imaged with confocal microscope. Arrows represent the BFA bodies and the bar indicates 10 μm.