Table 1.
Human Fat Depot (n=12) | SAT | VAT |
Glycerol output (G) in μmoles/g of AT/1h = lipolytic index | 0.44 ± 0.11 | 0.66 ± 0.23 |
Fatty acid output (FFA) in μmoles/g of AT/1h | 0.46 ± 0.19 | 1.04 ± 0.34 |
Free fatty acid versus glycerol output (FFA/G) in μmoles/g of AT/1h (without GNG, should be 3) | 1.05 ± 0.46 | 1.57 ± 0.48 |
[14C1|-pyruvate incorporation into triglycerides in nmoles/g of AT/1h = FFA re-esterification via GNG | 5.60 ± 3.29 | 8.11 ± 2.86 |
PEPCK-C specific activity in nmoles/mn/mg protein | 0.67 ± 0.21 | 1.35 ± 0.64 |
Glycerol and FFA release were measured in the culture medium and FFA re-esterification via glyceroneogenesis was appreciated by measuring the [14C1|-pyruvate incorporation into triglycerides and the PEPCK-C enzyme activity in subcutaneous and visceral fat explants from 12 lean women. The results are expressed as mean ± SEM. *(p < 0.05) between SAT and VAT mean values.