Fig. 4.

TNF-α treatment of 3T3-L1 adipocytes increases lipolysis that is enhanced by further depletion of FSP27 using siRNA. A: Glycerol released in medium was measured in 3T3-L1 adipocytes electroporated with scrambled (Scr) or FSP27 siRNA and treated with 10 ng/ml TNF-α for 16 h. A.U., arbitrary units. B: Western blot analysis using FSP27, perilipin (PLIN), and actin antibodies showing FSP27 and PLIN protein levels in control and under TNF-α-treated conditions. MW, molecular weight. C: Densitometric analysis of FSP27 and PLIN protein levels in siRNA or TNF-α-treated cells. Data are expressed as means ± SEM for six different experiments. KD, knock-down. P values were calculated using ANOVA; *, P < 0.05; **, P < 0.0005.