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. 2011 Feb;52(2):245–255. doi: 10.1194/jlr.M011346

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Copyright © 2011 by the American Society for Biochemistry and Molecular Biology, Inc.

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Fig. 4. — Biosynthesis of VLC-PUFAs in wild-type, Elovl2^+/−, and Elovl2^−/− mice. The composition of the testicular acyl-CoA pool was determined by extraction, derivatization, and HPLC analysis of acyl-etheno-CoAs. Carbon 17:0 istd is the internal standard acyl-CoA. A: Acyl-CoA composition of wild-type and Elovl2^−/− testes. Blue trace corresponds to Elovl2^+/+, and the red trace corresponds to Elovl2^−/− mice. *, indicates differences between the genotypes. B: Acyl-CoA composition of wild-type and Elovl2^+/− testes. Blue trace corresponds to Elovl2^+/+ mice, and the red trace corresponds to Elovl2^+/− mice. *, indicates differences between the genotypes. Results shown are pooled samples from 7 wild-type and heterozygous mice and 2 knockout animals.