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Thin-layer chromatogram of hydrolyzates of CHS with HCl. 1, intact CHS; 2, hydrolyzates before separation; 3, product with mono-saccharide residue (CHS-M); 4, product with di-saccharide residue (CHS-D); 5, product with tri-saccharide residue (CHS-T); 6, product with tetra-saccharide residue (CHS-Q); 7, remaining CHS. The plate was developed with chloroform-methanol-water (60:40:10, v/v/v). Spots were visualized with orcinol-sulfuric acid reagent.
