TABLE 3.
Chemical shifts and J1,2 coupling constants of the protons of the isolated glycosphingolipids in the anomeric regions
| CMS | Glc1 | |||||
|---|---|---|---|---|---|---|
| I | ||||||
| Chemical shifts (ppm) | 4.09 | |||||
| Coupling constants (Hz) | 7.8 | |||||
| CDS | Man1- | 4Glc1 | ||||
| II | I | |||||
| Chemical shifts (ppm) | 4.50 | 4.15 | ||||
| Coupling constants (Hz) | ∼1 | 7.8 | ||||
| nAtCTS | Fuc1- | 3Man1- | 4Glc1 | |||
| III | II | I | ||||
| Chemical shifts (ppm) | 4.79 | 4.52 | 4.15 | |||
| Coupling constants (Hz) | 3.7 | ∼1 | 7.8 | |||
| AtCTS | GlcNAc1- | 3Man1- | 4Glc1 | |||
| IV | II | I | ||||
| Chemical shifts (ppm) | 4.53 | 4.53 | 4.16 | |||
| Coupling constants (Hz) | 7.3 | ∼1 | 7.8 | |||
| nAtCTeS | GlcNAc1- | 2Fuc1- | 3Man1- | 4Glc1 | ||
| V | III | II | I | |||
| Chemical shifts (ppm) | 4.80 | 4.95 | 4.52 | 4.15 | ||
| Coupling constants (Hz) | 3.7 | 3.7 | ∼1 | 7.8 | ||
| AtCTeS | GalNAc1- | 4GlcNAc1- | 3Man1- | 4Glc1 | ||
| VI | IV | II | I | |||
| Chemical shifts (ppm) | 4.35 | 4.55 | 4.52 | 4.15 | ||
| Coupling constants (Hz) | 8.7 | 8.2 | ∼1 | 7.8 | ||
| CPS | GalNAc1-4 | (Fuc1-3) | GlcNAc1- | 3Man1- | 4Glc1 | |
| VI | III | IV | II | I | ||
| Chemical shifts (ppm) | 4.29 | 4.83 | 4.62 | 4.52 | 4.16 | |
| Coupling constants (Hz) | 8.7 | 3.7 | 6.4 | ∼1 | 7.8 | |
| CHS | GalNAc1-4 | (GlcNAc1- | 2Fuc1-3) | GlcNAc1- | 3Man1- | 4Glc1 |
| VI | V | III | IV | II | I | |
| Chemical shifts (ppm) | 4.31 | 5.01 | 5.01 | 4.64 | 4.52 | 4.15 |
| Coupling constants (Hz) | 8.2 | 2.7 | 2.7 | 7.3 | ∼1 | 7.8 |