Fig. 3.

TAG hydrolysis in Pnpla3 knockout (KO) mice. (A) TAG hydrolase activity was determined in cytosolic fractions of perigonadal WAT lysates from WT and KO mice (n = 6/group, male, 16–17 week-old, ad libitum-fed, chow diet) in the presence or absence of the PNPLA2/ATGL coactivator CGI-58 and/or the HSL-specific in­hibitor 76-0079. Similar results were obtained for BAT (data not shown). (B) Glycerol release over time from perigonadal WAT explants of WT and KO mice (n = 3/group, male, 8–10 week-old, ad libitum-fed, chow diet) in the absence (basal) or presence of 10 μM isoproterenol (stimulated, stim). Comparable results were obtained for NEFA release (data not shown). (C-D) mRNA expression of Pnpla2/ATGL (C) and HSL (D) in perigonadal WAT of WT and KO mice (n = 7–10/group, mixed gender, 7–8 week-old, ad libitum-fed, chow diet). Expression is normalized to 18S rRNA and expressed relative to gene expression in the WT control group. No significant differences were identified.