Fig. 1.
Structures of the fluorescent substrates used. A: Chemical structure of PLA1-specific substrate (N-{[6-(2,4-dinitrophenyl) amino] hexanoyl}-1-{4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-2-hexyl-sn-glycero-3-phosphoethanolamine; mw = 849). B: Chemical structure of PED6 substrate (N-{[6-(2,4-dinitrophenyl) amino]hexanoyl}-2-[4,4-difluoro-5,7-dimethyl-4-bora-3a,-4a-diaza-s-indacene-3-pentanoyl}-1-hexadecanoyl-sn-glycero-3-phosphoethanolamine; mw = 1136). Positions of the sn-1 and sn-2 cleavage sites are indicated for both substrates, and the BODIPY fluor and dinotrophenyl quencher is indicated on the PLA1 substrate.