Fig. 5.

PED6 substrate versus PLA₁ substrate turnover by EL, HL and LPL in stably expressing HEK293 cell lines. A and B: Cells stably expressing either murine EL, hHL or hLPL were assayed using either the PED6 (A) or PLA₁ (B) substrate. For both substrates, 30 min cell-based assays were run with increasing substrate concentrations. The level of expression for the three lipases in each cell line was comparable, based on qualitative Western blotting (data not shown). These triplicate assays represent one of three different experiments yielding identical results.