FIG. 9.

Detecting anti-HSP-90 antibodies after 8 week exposure to TCE and HgCl₂. SDS-PAGE was performed using recombinant HSP-90α as the source of protein. The protein was then immunoblotted using pooled detecting sera collected from the different treatment groups at 8 weeks, followed by a goat anti-mouse IgG antibody. This approach was repeated using individual or pairs of pooled sera (n = 3 or 4 per group), and the bar graph describes the percentage of these Western blots in which anti-HSP-90 was detected.