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. 2010 Jul 27;1(3):140–143. doi: 10.4161/trns.1.3.13191

Figure 1.

Figure 1

(A) Cartoon sketching of a telomeric genic unit composed of a subtelomeric promoter (61-29-37 repeats) and telomeric repeats. The arrow indicates the TERRA transcription start site. The yellow stars represent methyl groups added to promoter cytosines through concerted action of DNMT1 and DNMT3b. In these settings, telomere extension is favored. (B) Lack of DNMT1 and DNMT3b methyl transferase activities leads to increased telomere transcription, appearance of alternative TERRA start sites and telomere shortening. (C) Genomic DNA from HCT116 and DKO cells was digested with HinfI and RsaI, electrophoresed in 0.7% agarose gels and hybridized to radio-labeled telomeric probes according to standard protocols. Molecular weights are indicated on the left. Note that telomeres in DKO cells are shorter than in parental HCT116 cells.