CD45ROneg (naïve) or CD45RAneg (memory) T cells were stimulated with DC.Foxp3 as described in Materials and Methods. Responder T cells from DC-T cell cultures were re-stimulated (using the identical form of DC used in the primary stimulation) on day 5. On day 14, CD4+ and CD8+ T cells were analyzed for intracellular IL-4 and IFN-γ (A) or IL-17A (B) by flow cytometry. In panel C, CD8+ T cells harvested from day 14 cultures were analyzed for expression of intracellular granzyme B by flow cytometry. For each panel, similar data were obtained in 3 independent experiments. Transduction efficiency in DC.Foxp3: 67%, 43% and 67% in panels A-C, respectively.