Figure 2.

Production of IL-10 by CD5⁺ B cells is enhanced in mice treated with antibiotics. Splenic and lymph node cells from naïve C57BL/6 and mice treated orally with antibiotics for one week were sorted into CD19⁺B220⁺ B cells and subsequently into CD5⁺ and CD5⁻ by flow cytometry, and CD1d expression was compared (A). CD19⁺B220⁺CD5⁺ and CD19⁺B220⁺CD5⁻ cells were cultured for 12 h in RPMI complete media with 100 ng/ml of LPS. IL-10 secreted to the culture media was compared by cytokine ELISA (B). A representative analysis of two combined experiments for six mice/group is shown.