Figure 4.
AQP1 protein stability in isotonic and hypertonic conditions. (A) Cells were incubated in isotonic or hypertonic medium for 4 h, then metabolic labeling with [35S]methionine was performed as described. Samples were harvested at the designated times for immunoprecipitation of AQP1 as described. Samples were resolved on SDS/PAGE gels, and dried gels were exposed to x-ray film. (B) Samples treated as in A were analyzed by densitometry and are expressed as percentage of time 0 (n > 4 per group).